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Common Risk Group 1 Agents
Escherichia coli (cloning strains)
MC1061, K-12, MG1655, MC1061, DH5alpha, DH10b, AD494(DE3), NovaBlue, HMS174, Origami (DE3), B strain, Bl21, NEB T7 Express, Bl21(DE3) pLysS, B834(DE3) pLysS, Rosetta, HB101, JM110
Bacteria
Alcaligenes faecalis, Bacillus cereus, Bacillus mageterium, Bacillus subtilis, Clostridium sporogenes, Enterobacter aerogenes, Enterobactor cloacae, Kocuria rosea, Lactobacillus spp., Micrococcus luteus, Mycobacterium smegatis, Naelgleria sicca, Neisseria subflava, Penicillium notatum, Rhizopus stolonifera, Rhodospirillum rubrum, Serratia marcescens, Staphylococcus epidermidis, Streptococcus bovis, Streptococcus lactis
Native Plant Pathogens
Fusarium pseudograminearum, Fusarium oxysporum, Aphanomyces cochlioides, Potato virus X, Potato virus y, Phytophthora infestans, Pythium ultimum, Rhizoctonia solani, Wheat streak mosaic virus, Barley yellow dwarf luteovirus, Barley stripe mosaic virus, Aphanomyces euteiches
Yeast Saccharomyces cerevisiae
Cell lines Sf9 Insect cell lines, Rodent cell lines
Health Hazards
Host Range
Biological agents not known to cause disease in healthy adult humans. However, some RG1 biological agents can act as opportunistic pathogens in immunocompromised individuals and the young and elderly.
Medical Precautions/Treatment
Prophylaxis None.
Vaccines None.
Surveillance Monitor for symptoms.
MSU Requirements Report any exposures.
Laboratory Hazards
Laboratory Acquired Infections (LAIs)
Unknown
Supplemental References
Canadian MSDS
http://www.phac-aspc.gc.ca/lab-bio/res/psds-ftss/index-eng.php
BMBL https://www.cdc.gov/labs/bmbl/?CDC_AAref_Val=https://www.cdc.gov/labs/BMBL.html
Containment Requirements
BSL2
For all procedures involving known or potentially infected cultures.
ABSL2
For all procedures utilizing infected animals.
Spill Procedures
Small
Notify others working in the lab. Remove PPE and don new PPE. Cover area of the spill with absorbent material and add fresh 1:10 bleach:water. Allow 20 munutes (or as directed) of contact time. After 20 minutes, cleanup and dispose of materials.
Large
  • Immediately notify all personnel in the lab and clear all personnel from the area. Remove any contaminated PPE/clothing and leave the lab. 
  • Secure the area by locking doors, posting signage and guarding the area to keep people out of the space. 
For assistance, contact MSU's Biosafety Officer (406-994-6733) or Safety and Risk Management (406-994-2711).
Exposure Procedures
Mucous membrane
Flush eyes, mouth, or nose for 5 minutes at eyewash station.
Other Exposures
Wash area with soap and water for 5 minutes.
Reporting
Immediately report incident to supervisor, complete a First Report of Injury form, and submit to Safety and Risk Management.
Medical Follow-up
During business hours: Bridger Occupational Health 3406 Laramie Drive. Weekdays 8am -6pm.  Weekends 9am-5pm
After business hours: Bozeman Deaconess Hospital Emergency Room 915 Highland Blvd Bozeman, MT
 
Viability
Disinfection
Susceptible to bleach, 70% ethanol, and other disinfectants as outlined by the EPA.
Inactivation
Inactivated by moist heat (15 minutes at 121°C) and dry heat (1 hour at 160-170°C).
Personal Protective Equipment (PPE)
Minimum PPE Requirements
Lab coat, disposable gloves, safety glasses, closed toed shoes, long pants
Additional Precautions
 Additioanl PPE may be required depending on lab specific SOPs and IBC Protocol.