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Characteristics
Morphology HSV-1 and HSV-2 are members of the Herpesviridea family. Are double stranded DNA viruses enclosed within an icosahedral capsid.
Disease

HSV-1: Primarily cold sores, eye infections, CNS infections.

HSV-2: primarily genital sores.
Zoonosis
none.
Health Hazards
Host Range
Humans 
Modes of Transmission  Direct contact with infected secretions or mucous membranes or skins with lesions
Signs and Symptoms  Cold sores: Mainly caused by HSV-1. Gingivostomatitis, fever, sore throat, mucosal edema, and painful lesions Genital herpes: Sexually transmitted disease mainly caused by HSV-2. Bilateral, painful, and extensive genital ulcers, which heal without scarring within 12 days.
Infectious Dose unknown
Incubation Period  1 to 26 days.
Medical Precautions/Treatment
Prophylaxis None available.
Vaccines None available.
Treatment Antiviral drugs like acyclovir, foscarnet valacyclovir, famciclovir, and penciclovir.
Surveillance Monitor for symptoms. Viral culture or PCR is used to detect presence of viral infection.
MSU Requirements  Report any exposures
Laboratory Hazards
Laboratory Acquired Infections (LAIs) None.
 Sources Virus is shed from saliva, cervix, and urethra. Cultures, frozen stocks, other samples described in IBC protocol.
Supplemental References
Canadian MSDS: http://www.phac-aspc.gc.ca/lab-bio/res/psds-ftss/index-eng.php
BMBL
 https://www.cdc.gov/labs/bmbl/?CDC_AAref_Val=https://www.cdc.gov/labs/BMBL.html
CDC https://www.cdc.gov/herpes/about/?CDC_AAref_Val=https://www.cdc.gov/std/herpes/stdfact-herpes.htm
NIH Guidelines https://osp.od.nih.gov/wp-content/uploads/NIH_Guidelines.pdf
Risk Group & Containment Requirements
Risk Group 2

Agents that are associated with human disease which is rarely serious and for which preventive or therapeutic interventions are often available.
BSL2
For all procedures involving suspected or known infectious specimen or cultures.
ABSL2
For all procedures utilizing infected animals.
Spill Procedures
Small
Notify others working in the lab. Remove PPE and don new PPE. Cover area of the spill with absorbent material and add fresh 1:10 bleach:water. Allow 20 munutes (or as directed) of contact time. After 20 minutes, cleanup and dispose of materials.
Large
  • Immediately notify all personnel in the lab and clear all personnel from the area. Remove any contaminated PPE/clothing and leave the lab. 
  • Secure the area by locking doors, posting signage and guarding the area to keep people out of the space. 
For assistance, contact MSU's Biosafety Officer (406-994-6733) or Safety and Risk Management (406-994-2711).
Exposure Procedures
Mucous membrane
Flush eyes, mouth, or nose for 5 minutes at eyewash station.
Other Exposures
Wash area with soap and water for 5 minutes.
Reporting
Immediately report incident to supervisor, complete a First Report of Injury form, and submit to Safety and Risk Management.
Medical Follow-up
During business hours: Bridger Occupational Health 3406 Laramie Drive. Weekdays 8am -6pm.  Weekends 9am-5pm
After business hours: Bozeman Deaconess Hospital Emergency Room 915 Highland Blvd Bozeman, MT
Viability
Disinfection Susceptible to 1:10 bleach:water, 70 % ethanol
Inactivation Inactivated by moist heat (15 minutes at 121oC) and dry heat (1 hour at 170oC). 
Survival Outside Host
Survives outside host on dry inanimate surfaces (hours to weeks).
Personal Protective Equipment (PPE)
Minimum PPE Requirements
Lab coat, disposable gloves, safety glasses, closed toed shoes, long pants
Additional Precautions
 Additioanl PPE may be required depending on lab specific SOPs and IBC Protocol.