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Non exotic strains: VSV-Indiana 1 serotype strains (e.g. Glasgow, MuddSummers, Orsay, San Juan) and VSV-New Jersey serotype strains (e.g. Ogden, Hazelhurst)

Characteristics
Morphology Bullet-shaped that is an enveloped single stranded negative sense RNA virus.
Disease

Flu-like respiratory infection. Primarily affects horses, cattle, and other animals; but can infect humans.
Zoonosis

Yes, through direct contact with infected animals or through the bite of an infected sand fly.
Health Hazards
Host Range Humans, animals, and sand flies.
Modes of Transmission  Transmitted by sand fly bite.
Signs and Symptoms  High fever, with subsequent flu-like symptoms including malaise, headaches, nausea, arthralgia, fatigue, and muscle pain.
Infectious Dose Unknown
Incubation Period  30 hours to 6 days.
Medical Precautions/Treatment
Prophylaxis None available.
Vaccines None available.
Treatment No specific treatment.
Surveillance Monitor for syptoms.
MSU Requirements  Report any exposures.
Laboratory Hazards
Laboratory Acquired Infections (LAIs) 13 cases prior to 1980.
 Sources Blood, throat secretions, saliva, exudates, or epithelial cells from open vesicles. Cultures, frozen stocks, other samples described in IBC protocol.
Supplemental References
Canadian MSDS http://www.phac-aspc.gc.ca/lab-bio/res/psds-ftss/index-eng.php
BMBL
 https://www.cdc.gov/labs/bmbl/?CDC_AAref_Val=https://www.cdc.gov/labs/BMBL.html
CDC
  
NIH Guidelines
 https://osp.od.nih.gov/wp-content/uploads/NIH_Guidelines.pdf
Risk Group & Containment Requirements
Risk Group 2 

Agents that are associated with human disease which is rarely serious and for which preventive or therapeutic interventions are often available.
BSL2
For all procedures involving suspected or known infectious specimen or cultures.
ABSL2
For all procedures utilizing infected animals.
Spill Procedures
Small
Notify others working in the lab. Remove PPE and don new PPE. Cover area of the spill with absorbent material and add fresh 1:10 bleach:water. Allow 20 munutes (or as directed) of contact time. After 20 minutes, cleanup and dispose of materials.
Large
  • Immediately notify all personnel in the lab and clear all personnel from the area. Remove any contaminated PPE/clothing and leave the lab. 
  • Secure the area by locking doors, posting signage and guarding the area to keep people out of the space. 
For assistance, contact MSU's Biosafety Officer (406-994-6733) or Safety and Risk Management (406-994-2711).
Exposure Procedures
Mucous membrane
Flush eyes, mouth, or nose for 5 minutes at eyewash station.
Other Exposures
Wash area with soap and water for 5 minutes.
Reporting
Immediately report incident to supervisor, complete a First Report of Injury form, and submit to Safety and Risk Management.
Medical Follow-up
During business hours: Bridger Occupational Health 3406 Laramie Drive. Weekdays 8am -6pm.  Weekends 9am-5pm
After business hours: Bozeman Deaconess Hospital Emergency Room 915 Highland Blvd Bozeman, MT
Viability
Disinfection Susceptible to 1:10 bleach:water, 70 % ethanol
Inactivation Inactivated by moist heat (15 minutes at 121° C) and dry heat (1 hour at 160-170° C)
Survival Outside Host Can survive for 3 to 4 days in saliva on milking pails, barns and hay.
Personal Protective Equipment (PPE)
Minimum PPE Requirements
Lab coat, disposable gloves, safety glasses, closed toed shoes, long pants
Additional Precautions
 Additioanl PPE may be required depending on lab specific SOPs and IBC Protocol.